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KMID : 0861020180330020069
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Korea Journal of Herbology
2018 Volume.33 No. 2 p.69 ~ p.77
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Effects for the New Formulation of Daesiho-tang on adipocyte development and differentiation in 3T3-L1
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Choi Hye-Min
Kim Se-Jin
Moon Sung-Ok
Lee Ji-Beom
Lee Ha-Young
Kim Jong-Beom
Lee Hwa-Dong
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Abstract
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Objectives : Daesiho-tang (DSHT) has been widely used in the treatment of cerebral infarct in traditional medicine. However, there was not report on the anti-obesity-related diseases efficacy of DSHT. In this study, we investigated the effects for the new formulation of DSHT, on the adipocyte differentiation cycle in 3T3-L1 cells.
Methods : 3T3-L1 cells were treated with DSHT (50, 100, 200 ㎍/㎖) during differentiation for 6 days. Also, the inhibitory effect of DSHT against 3T3-L1 adipogenesis was evaluated in various stage of adipogenesis such as early (0-2day), intermediate (2-4day), and terminal stage (4-6day). The accumulation of lipid droplets was determined by Oil Red O staining. and, the expressions of genes related to adipogenesis were measured by RT-PCR and Western blot analyses.
Results : DSHT showed inhibitory activity on adipocyte differentiation at 3T3-L1 preadipocytes without affect cell toxicity as assessed by measuring fat accumulation and adipogenesis. In addition, DSHT significantly reduced the expression levels of several adipocyte marker genes including proliferator activated receptor-γ (PPAR-γ) and CCAAT/ enhancer-binding protein-α (C/EBP-α). Also, the anti-adipogenic effect of DSHT was strongly limited in the intermediate (2-4 day), terminal stage (4-6 day) of 3T3-L1 adipogenesis. In addition, the DSHT treatment down- regulated mRNA expression levels of PPAR-γ,, C/EBP-α in mature 3T3-L1 adipocytes.
Conclusions : These results suggest that, the ability of DSHT has inhibited overall adipogenesis and lipid accumulation in the 3T3-L1 cells. The new formulation of DSHT may be a promising medicine for the treatment of obesity and related metabolic disorders.
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KEYWORD
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Daesiho-tang, Traditional medicine, Anti-obesity, 3T3-L1 preadipocyte, differentiation cycle
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